Ever since the 'cultural turn' in Translation Studies it has been commonplace to state that translation is an act of cultural mediation. However, the concept of culture as such has remained elusive. A number of questions remain unanswered: How can we define a culture? What kind of empirical evidence is needed to prove the existence of a particular culture? Looking for answers, this article starts with the author's own attempt at conceptualizing translators’ work in the European Commission by defining the EU institutions as a (multilingual and institutional) culture of its own. Responses to this model convey varying views of the concept of culture. By analyzing and contextualizing these responses this article aims to provide some answers to the question of what kind of a construction culture is. The results of the analysis are then used to reflect on recent developments in Translation Studies.